LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2618..2714) /label=attL2 /note="attL2 (c)" misc_signal complement(2729..2748) /note="T7 Promoter/priming site (c)" primer_bind 2756..2772 /note="M13 Reverse priming site" gene 2885..3694 /note="Kanamycin resistance gene" rep_origin 3815..4488 /note="pUC origin" PCR_frame 669..2618 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2638) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2635) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2626..4491,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2596..2616 /note="TEV site" source 673..2595 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:19707 IMAGE:3532913" /tissue_type="Muscle, rhabdomyosarcoma" /clone_lib="NIH_MGC_17" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2595 /gene="PDIA4" /gene_synonym=ERP70 /gene_synonym=ERP72 /db_xref="GeneID:9601" /db_xref="HGNC:30167" CDS 673..2595 /dnas_title="protein disulfide isomerase family A, member 4" /gene="PDIA4" /gene_synonym=ERP70 /gene_synonym=ERP72 /codon_start=1 /product="protein disulfide isomerase family A, member 4" /protein_id="AAH11754.1" /db_xref="GI:15079928" /db_xref="GeneID:9601" /db_xref="HGNC:30167" /translation="MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEE EEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYE KIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEI VAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEK AAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIV DYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDY KFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKY ALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTF AIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKG KLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYN SLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDL EHLSKFIEEHATKLSRTKEEL" misc_feature 673..2595 /note="PDIA4 coding region" misc_difference 2328..2328 /gene="PDIA4" /gene_synonym=ERP70 /gene_synonym=ERP72 /note="'A' in cDNA is 'G' in the human genome; no amino acid change. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgaggcc ccggaaagcc ttcctgctcc tgctgctctt ggggctggtg 721 cagctgctgg ccgtggcggg tgccgagggc ccggacgagg attcttctaa cagagaaaat 781 gccattgagg atgaagagga ggaggaggag gaagatgatg atgaggaaga agacgacttg 841 gaagttaagg aagaaaatgg agtcttggtc ctaaatgatg caaactttga taattttgtg 901 gctgacaaag acacagtgct gctggagttt tatgctccat ggtgtggaca ttgcaagcag 961 tttgctccgg aatatgaaaa aattgccaac atattaaagg ataaagatcc tcccattcct 1021 gttgccaaga tcgatgcaac ctcagcgtct gtgctggcca gcaggtttga tgtgagtggc 1081 taccccacca tcaagatcct taagaagggg caggctgtag actacgaggg ctccagaacc 1141 caggaagaaa ttgttgccaa ggtcagagaa gtctcccagc ccgactggac gcctccacca 1201 gaagtcacgc ttgtgttgac caaagagaac tttgatgaag ttgtgaatga tgcagatatc 1261 attctggtgg agttttatgc cccatggtgt ggacactgca agaaacttgc ccccgagtat 1321 gagaaggccg ccaaggagct cagcaagcgt tctcctccaa ttcccctggc aaaggtcgac 1381 gccaccgcag aaacagacct ggccaagagg tttgatgtct ctggctatcc caccctgaaa 1441 attttccgca aaggaaggcc ttatgactac aacggcccac gagaaaaata tggaatcgtt 1501 gattacatga tcgagcagtc cgggcctccc tccaaggaga ttctgaccct gaagcaggtc 1561 caggagttcc tgaaggatgg agacgatgtc atcatcatcg gggtctttaa gggggagagt 1621 gacccagcct accagcaata ccaggatgcc gctaacaacc tgagagaaga ttacaaattt 1681 caccacactt tcagcacaga aatagcaaag ttcttgaaag tctcccaggg gcagttggtt 1741 gtaatgcagc ctgagaaatt ccagtccaag tatgagcccc ggagccacat gatggacgtc 1801 cagggctcca cccaggactc ggccatcaag gacttcgtgc tgaagtacgc cctgcccctg 1861 gttggccacc gcaaggtgtc aaacgatgct aagcgctaca ccaggcgccc cctggtggtc 1921 gtctactaca gtgtggactt cagctttgat tacagagctg caactcagtt ttggcggagc 1981 aaagtcctag aggtggccaa ggacttccct gagtacacct ttgccattgc ggacgaagag 2041 gactatgctg gggaggtgaa ggacctgggg ctcagcgaga gtggggagga tgtcaatgcc 2101 gccatcctgg acgagagtgg gaagaagttc gccatggagc cagaggagtt tgactctgac 2161 accctccgcg agtttgtcac tgctttcaaa aaaggaaaac tgaagccagt catcaaatcc 2221 cagccagtgc ccaagaacaa caagggaccc gtcaaggtcg tggtgggaaa gacctttgac 2281 tccattgtga tggaccccaa gaaggacgtc ctcatcgagt tctacgcacc atggtgcggg 2341 cactgcaagc agctagagcc cgtgtacaac agcctggcca agaagtacaa gggccaaaag 2401 ggcctggtca tcgccaagat ggacgccact gccaacgacg tccccagcga ccgctataag 2461 gtggagggct tccccaccat ctacttcgcc cccagtgggg acaaaaagaa cccagttaaa 2521 tttgagggtg gagacagaga tctggagcat ttgagcaagt ttatagaaga acatgccaca 2581 aaactgagca ggaccgaaaa cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTTGG 2641 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 2701 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 2761 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 2821 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 2881 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 2941 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 3001 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 3061 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 3121 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 3181 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 3241 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 3301 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 3361 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 3421 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 3481 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 3541 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 3601 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 3661 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 3721 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 3781 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 3841 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 3901 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 3961 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 4021 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 4081 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 4141 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 4201 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 4261 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 4321 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 4381 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 4441 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //